Culture media compositions free of fetal bovine serum

C - Chemistry – Metallurgy – 12 – N

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C12N 1/00 (2006.01) C12N 1/10 (2006.01)

Patent

CA 2555869

A cell culture growth media free of Fetal Bovine Serum for use with parasitic organisms. The media includes calcium chloride, sodium bicarbonate, potassium chloride, sodium chloride, monosodium phosphate, glucose, hepes, ferric nitrate, magnesium sulfate, tricine, d-ribose, 2-deoxy ribose, adenosine-5- triphosphate (ATP), 2-deoxyadenylic acid (d-AMP), 5'-thymidylic acid (TMP), 2'- deoxyicitidine-5 monophosphate (d, 2'-deoxyuridine-5~monophosphate (d, 2'- deoxyguanilic Acid (d-GMP), aspartic acid, glutamic acid, l-alanine, arginine, carnosine, cysteine, cystine, glutamine, glycine, histidine, iso-leucine, leucine, lysine, methionine, ornitine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, ascorbic acid, biotine (H), carnitine, cholecalciferol, choline chloride, cyanocobalamine (B12), ergocalciferol, folic acid, myo-inositol, menadione, nicotinamide, PABA, panthotenato, pyridoxal, pyridoxamine, pyridoxine, retinol (A), riboflavine (B2), Thiamine (B1), 6,8 Thiotic acid, alfa-tocoferol, 3-phytylmenadione (K1), tetrahydrofolic acid, hemin from procine, and nanopure water.

Milieux de croissance de cultures cellulaires exempts de sérum bovin foetal et conçus pour être utilisés avec des organismes parasitaires. Ce milieu comprend chlorure de calcium, bicarbonate de sodium, chlorure de potassium, chlorure de sodium, phosphate monosodique, glucose, hepes, nitrate ferrique, sulfate de magnésium, tricine, d-ribose, 2-déoxy ribose, adénosine-5-triphosphate (ATP), acide 2-déoxyadenylique (d-AMP), acide 5'-thymidylique (TMP), 2'-déoxyicitidine-5 monophosphate (d, 2'-déoxyuridine-5-monophosphate (d, acide 2'-déoxyguanilique (d-GMP), acide aspartique, acide glutamique, l-alanine, arginine, carnosine, cystéine, cystine, glutamine, glycine, histidine, iso-leucine, leucine, lysine, méthionine, ornitine, phénylalanine, proline, sérine, thréonine, tryptophane, tyrosine, valine, acide ascorbique, biotine (H), carnitine, cholécalciférol, chlorure de choline, cyanocobalamine (B12), ergocalciférol, acide folique, myo-inositol, ménadione, nicotinamide, PABA, panthoténato, pyridoxal, pyridoxamine, pyridoxine, rétinol (A), riboflavine (B2), Thiamine (B1), acide 6,8 thiotique, alfa-tocoférol, 3-phytylménadione (K1), acide tétrahydrofolique, hémine de procine, et eau nanopure.

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