C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01)
Patent
CA 2421436
The invention relates to a novel culture medium and a method for the identification of gram-negative microorganisms based on the differentiation of said microorganisms by the appearance of 10 different colors in the colonies, which may be regular or irregular, and halos of at least 5 different colors and sizes. Said medium comprises a mixture of components favoring the appearance of halos of different colors and sizes and consists of siliceous earth, skim milk, starches and activated carbon. The medium according to the invention also comprises a mixture of nutritional bases, substances ensuring the appearance of different colorations in the colonies, substances ensuring inhibition of gram-positive microorganisms and substances providing the necessary solid matrix for the growth and development of the colonies.
L'invention concerne un milieu de culture et une méthode destinés à l'identification d'organismes gram-négatifs se basant sur la différenciation de ces derniers par apparition d'au moins 10 couleurs différentes des colonies, qui peuvent être régulières ou irrégulières, et de halos d'au moins 5 différentes colorations et dimensions. Ce milieu comprend un mélange de composants qui favorisent l'apparition de halos de différentes couleurs et dimensions, ce mélange étant constitué de terre siliceuse, de lait écrémé, d'amidons et de charbon activé. En outre, ce milieu comprend un mélange de bases nutritives, de substances qui garantissent l'apparition de différentes colorations dans les colonies, de substances qui garantissent l'inhibition des micro-organismes gram-positifs et de substances qui fournissent la matrice solide nécessaire à la croissance et au développement des colonies.
Quesada Muniz Vivian de Jesus
Rodriguez Martinez Claudio
Zhurbenko Raisa
Centro Nacional de Biopreparados
Macrae & Co.
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