Culture medium composition, culture method, and myoblasts...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/077 (2010.01) C12N 5/0775 (2010.01) A61K 35/34 (2006.01)

Patent

CA 2509642

The invention concerns a composition of culture medium of progenitor/stem cells derived from muscular tissues containing serum and/or serum fraction of human origin and/or of animal origin of insulin or a derivative thereof, and one or several compound(s) selected among the class of antioxidants and/or of vitamins. The invention also concerns a method for culturing progenitor/stem cells, a method for producing myoblasts capable of being used as cellular/genetic therapy product. The invention aims at optimizing the production of myoblasts from progenitor/stem cells.

La présente invention se rapporte à une composition de milieu de culture de cellules progénitrices/souches issues de tissus musculaires contenant du sérum et/ou de la fraction sérique d'origine humaine et/ou d'origine animale de l'insuline ou un dérivé de celle-ci, et un ou plusieurs composé(s) choisi(s) parmi la classe des antioxydants et/ou des vitamines. Elle propose également un procédé de culture des cellules progénitrices/souches, un procédé de production de myoblastes pouvant être utilisés comme produit de thérapie cellulaire/génique. Cette invention a pour objectif d'optimiser la production en myoblastes à partir de cellules progénitrices/souches.

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