Culture medium for enhanced heterologous protein expression

Details Culture medium for enhanced heterologous protein expression Culture medium for enhanced heterologous protein expression

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C07K 14/205 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2439096

A cell culture medium comprising glycerol or glucose, an ammonium salt, sodium polyphosphate, a magnesium salt, a potassium salt and trace elements, and having a pH between 5.5 and 8Ø The medium is particularly suitable for culturing E.coli for the expression of heterologous proteins (e.g., NAP from H.pylori).

L'invention concerne un milieu de culture cellulaire, qui contient un glycérol ou glucose, un sel d'ammonium, un polyphosphate de sodium, un sel de magnésium, un sel de potassium et un microconstituant; et présente un pH compris entre 5,5 et 8,0. Ce milieu convient particulièrement pour la culture de E.coli destinée a l'expression de protéines hétérologues (e.g., NAP issues de H.pylori).

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