Culture medium for epithelial stem cells and organoids...

Details Culture medium for epithelial stem cells and organoids... Culture medium for epithelial stem cells and organoids...

C - Chemistry, Metallurgy

12

N

C12N 5/073 (2010.01)

Patent

CA 2751332

The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.

La présente invention concerne un procédé de culture de cellules souches épithéliales, des fragments de tissus isolés comprenant lesdites cellules souches épithéliales, ou des cellules d'adénomes, et de culture de cellules ou de fragments en présence d'un inhibiteur de la protéine morphogénétique osseuse (BMP), d'un facteur de croissance mitogénique, et d'un agoniste Wnt lors de la culture des cellules souches épithéliales et des fragments de tissus isolés. L'invention concerne en outre un milieu de culture cellulaire comprenant un inhibiteur de la BMP, un facteur de croissance mitogénique, et un agoniste Wnt, l'utilisation dudit milieu de culture, et les organoïdes crypto-villositaires, les organoïdes gastriques et les organoïdes pancréatiques qui sont formés dans ledit milieu de culture.

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