Culture medium to improve the purity of myoblast culture

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/08 (2006.01) A61K 31/198 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2354978

The present invention relates to an improved culture medium that allows the preferential growth of myoblasts over fibroblasts. Specifically, a culture medium disclosed by Ham et al. was modified by substituting a part or the totality of L-valine with D-valine. This improved culture medium allows the selection of primary myoblasts from fibroblasts, since the fibroblasts cannot use D-valine while the myoblasts can.

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