C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/0797 (2010.01)
Patent
CA 2756283
A method for differentiating a human differentiated cell-derived pluripotent stem cell into a neural stem cell is provided, which includes the steps of: making an embryoid body from the human differentiated cell-derived pluripotent stem cell; and culturing the embryoid body in a medium containing LIF to differentiate into a neural stem cell, so that, when the neural stem cell is allowed to differentiate in vitro after multiple subculturing of the neural stem cell, it differentiate mainly into neurons but substantially not into glial cells.
L'invention porte sur un procédé pour la différenciation d'une cellule souche pluripotente issue d'une cellule humaine différenciée en une cellule souche neurale, qui comprend les étapes consistant à : fabriquer un corps embryoïde à partir de la cellule souche pluripotente issue d'une cellule humaine différenciée ; et effectuer la culture du corps embryoïde dans un milieu contenant du LIF pour la différenciation en une cellule souche neurale, de façon à ce que, lorsqu'on laisse la cellule souche neurale se différencier in vitro après de multiples sous-cultures de la cellule souche neurale, elle se différencie principalement en neurones et pratiquement pas en cellules gliales.
Nakamura Masaya
Okada Yohei
Okano Hideyuki
Keio University
Sim & Mcburney
LandOfFree
Culture method of embryoid bodies and/or neural stem cells... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Culture method of embryoid bodies and/or neural stem cells..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Culture method of embryoid bodies and/or neural stem cells... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1383182