C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/08 (2006.01) A61K 35/14 (2006.01) A61K 35/18 (2006.01) A61K 38/00 (2006.01) A61K 38/20 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2016821
-24- TITLE IMPROVED CULTURE OF HUMAN TUMOR-INFILTRATING LYMPHOCYTES BY COMBINING GAS PERMEABLE BAGS AND A PRIMARY CULTURE CONTAINER ABSTRACT OF THE DISCLOSURE There is disclosed a process for the enhanced expansion and maintenance of human tumor-infiltrating lymphocytes (TIL). The process comprises placing a human tumor sample in a primary culture container and maintaining the sample in the primary culture container for a period of time before transferring a substantial amount of the primary culture to a bulk culture container. The primary culture cells and/or conditioned medium retained in the primary culture container are periodically used to supplement the bulk culture. Both primary and bulk cultures are maintained and expanded by periodic addition of a suitable culture medium as needed.
Nahapetian Ara T.
Wiedmeyer Jeriulou A.
E.i. Du Pont de Nemours And Company
Nahapetian Ara T.
Sim & Mcburney
Terumo Kabushiki Kaisha
Wiedmeyer Jeriulou A.
LandOfFree
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