C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) C12M 3/00 (2006.01) C12M 3/04 (2006.01) C12M 3/06 (2006.01)
Patent
CA 2105419
Abstract There is a known culture vessel, similar to a roller bottle, for cell cultures which, forming a supply chamber, contains a nutrient medium in which is arranged at least one production chamber which receives the cell cultures and is bathed by the nutrient medium, and is separated from the nutrient medium by a dialysis membrane through which nutrients are transported from the supply chamber into the production chamber, and with a gas feed and discharge system into the supply chamber. In order to make available, on this basis, a culture vessel for generating cell cultures with a high cell density which is economical to manufacture and easy to handle, and in which the danger of infections is diminished, it is proposed that there be provided as a gas feed and discharge system for the gases required and produced during cell culturinq a gas exchange membrane that forms at least a part of the outer wall of the culture vessel and whose total surface area, material, and thickness are selected so that the permeability to oxygen is at least 0.01 mg/h for each 107 cells. Also known are methods for cell culturing wherein a nutrient medium that bathes a production chamber that is sealed on all sides and contains the cell culture being cultured is placed in a culture vessel and a continuous rolling motion is imparted to the culture vessel. In order to indicate, on this basis, a method that is easy to implement and with which high cell densities with concurrently high vitality can be generated in a short time, the production chamber is filled with the cell culture being cultured in such a way that it contains an air bubble whose volume occupies 10% of the volume of the production chamber, and a tumbling movement of the culture vessel is superimposed on the rolling movement in such a way that the air bubble moves in the cell culture.
Heraeus Instruments Gmbh & Co. Kg
Macrae & Co.
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