Culturing method and culture apparatus

Details Culturing method and culture apparatus Culturing method and culture apparatus

C - Chemistry, Metallurgy

12

N

195/47, 195/89

C12N 5/02 (2006.01) C12M 1/00 (2006.01) C12M 3/02 (2006.01) C12N 5/04 (2006.01) C12P 17/18 (2006.01)

Patent

CA 1336078

Disclosed is a culturing method which comprises culturing a cell in a liquid medium in culture tanks to produce a useful substance. At least two tanks communicating with each other are used. A first culture tank's condition is set suitable for the growth of the cell and a second culture tank's condition is set suitable for the production of the useful substance. A culture solution constituting a culture medium suited to the growth of the cell is continuously or intermittently fed to the first culture tank. A part of the culture solution as a liquid medium stored in the first culture tank is continuously or intermittently transferred together with the cell and contained in the culture solution to the second culture tank. A part of the culture solution as a culture medium stored in the second culture tank is continuously or intermittently drawn out from the tank. A culture apparatus for carrying out the method is also disclosed.

573268

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