Customized oligonucleotide microchips that convert multiple...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2363073

This invention relates to using customized oligonucleotide microchips as biosensors for the detection and identification of the nucleic acids specific for different genes, organisms and/or individuals in the environment, in food and in biological samples. The microchips are designed to convert multiple bits of genetic information into simpler patterns of signals that are interpreted as a unit. Because of an improved method of hybridizing oligonucleotides from samples to microchips, microchips are reusable and transportable. For field study, portable laser or bar code scanners are suitable.

L'invention concerne l'utilisation de micropuces à oligonucléotides, en tant que biocapteurs destinés à la détection et l'identification d'acides nucléiques spécifiques de différents gènes, organismes et/ou individus, dans l'environnement et dans des échantillons alimentaires et biologiques. Ces micropuces sont conçues pour convertir plusieurs binaires d'informations génétiques en motifs plus simples de signaux qui sont interprétés en tant qu'unité. Etant donné qu'il existe un procédé perfectionné d'hybridation d'oligonucléotides à partir des échantillons pour obtenir des micropuces, ces micropuces sont réutilisables et transportables. Pour une étude sur le terrain, des lecteurs laser ou de code barres, portatifs, sont appropriés.

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