Cyanine dyes with high-absorbance cross section as donor...

Details Cyanine dyes with high-absorbance cross section as donor... Cyanine dyes with high-absorbance cross section as donor...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2267654

Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the transfer by emitting fluorescent light for detection. The cyanine dyes impart an unusually high sensitivity to the labels thereby improving their usefulness in a wide variety of biochemical procedures, particularly nucleic acid sequencing, nucleic acid fragment sizing, and related procedures.

Colorants à base de cyanine, en tant que fluorophores donneurs dans les marqueurs de transfert d'énergie, selon le principe d'absorption de l'énergie lumineuse par un fluorophore donneur, avec transfert sur un fluorophore accepteur qui réagit à ce transfert en émettant une lumière fluorescente aux fins de détection. Les colorants considérés confèrent une sensibilité inhabituellement élevée aux marqueurs, améliorant l'efficacité desdits marqueurs dans une large gamme de procédures biochimiques, en particulier pour le séquençage nucléotidique, la détermination de la taille des fragments d'acides nucléiques, et autres procédures connexes.

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