Cyclic nucleotide phosphodiesterase and a method for...

Details Cyclic nucleotide phosphodiesterase and a method for... Cyclic nucleotide phosphodiesterase and a method for...

C - Chemistry, Metallurgy

12

N

C12N 9/16 (2006.01)

Patent

CA 2153265

A novel cyclic nucleotide phosphodiesterase, which is different from known isozyme families, is isolated and purified from rat cerebrum. This enzyme has the following physicochemical properties (1) to (3). (1) The enzyme acts on cAMP to form 5' -AMP and on cGMP to form 5' -GMP; (2) The Km values for cAMP and cGMP are 0.11 µM and 1.78 µM, respectively, as indicating substrate specificity; and (3) the molecular weight is approximately 298000.

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