Cyclin-dependent kinase inhibitors as plant growth regulators

Details Cyclin-dependent kinase inhibitors as plant growth regulators Cyclin-dependent kinase inhibitors as plant growth regulators

A - Human Necessities

01

H

A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2235978

Cyclin-dependent kinase (CDK) inhibitor genes encode low molecular weight proteins which have important functions in cell cycle regulation, development and perhaps also in tumorigenesis (Sherr and Roberts, 1995; Harper and Elledge, 1996). The first plant CDK inhibitor gene ICKI was recently identified from Arabidopsis thaliana (Wang et al., 1997). Although the C-terminal domain of ICK1 contained an important consensus sequence with the mammalian CDK inhibitor p27 KiP1, the remainder of the deduced ICK1 sequence showed little similarity to any known CDK inhibitors. In vitro assays showed that recombinant ICK1 exhibited unique kinase inhibitory properties. In the present study we characterized ICK1 in terms of its gene structure, its interaction with both Arabidopsis thaliana Cdc2a and cyclin 83 and its induction by the plant growth regulator, abscisic acid (ABA). ICK1 was expressed at a relatively low and uniform level in the tissues surveyed. However, ICKI was induced by, ABA, and along with ICKI induction there was a decrease in Cdc2-associated histone H1 kinase activity. These results suggest a molecular mechanism by which plant cell division might be inhibited by ABA. ICKI clones were also identified from independent yeast two-hybrid screens using the cyclin .delta.3 construct. The implication that ICK1 protein could interact with both Cdc2a and cyclin .delta.3 was confirmed by in vitro binding assays. Furthermore, deletion analysis indicated that different regions of ICK1 are required for the interactions with Cdc2a and cyclin .delta.3. These results provide mechanistic basis for understanding the role of CDK inhibitors in cell cycle regulation in plant cells.

Affiliated with

Also associated with

Rating

Cyclin-dependent kinase inhibitors as plant growth regulators does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cyclin-dependent kinase inhibitors as plant growth regulators, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cyclin-dependent kinase inhibitors as plant growth regulators will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1670214