Cyclin-dependent kinase inhibitors as plant growth regulators

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2256121

The invention provides methods for using cyclin-dependent kinase (CDK) inhibitor genes, or anti-sense constructs complementary to such genes, to modify the growth and development of plant cells and organs. The invention provides methods of modifying the development of plant cells and plants by transforming plant cells with nucleic acids encoding cyclin-dependent kinase inhibitor polypeptides, or anti-sense constructs complementary to such nucleic acids, to produce transformed plant cells, and then culturing the plant cells or regenerating a plant under conditions wherein the cyclin-dependent kinase inhibitor, or the anti-sense construct, is expressed. A variety of CDK inhibitor genes, and corresponding anti-sense constructs, are disclosed for use in a variety of plants. The nucleic acid encoding the cyclin-dependent kinase inhibitor may be operably linked to a tissue-specific promoter. Another aspect of the invention provides modified transgenic plants and plant tissues. The invention also provides methods of identifying nucleic acids that encode cyclin-dependent kinase inhibitors that are active in plants to modify the development of the plants.

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