Cyclodextrin glycosyl transferases for producing...

Details Cyclodextrin glycosyl transferases for producing... Cyclodextrin glycosyl transferases for producing...

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2199904

CGTases, which, when converting starch or starch-like substrates to CD, produce .gamma.-CD to an increased extent and still exhibit at least 60% of the specific total CGTase activity of the starting CGTase which was used for preparing the enzyme concerned. The amino acid sequences differ from the amino acid sequences of known CGTases by the deletion of at least one amino acid from the region from amino acid position 155 up to and including amino acid position 195, where position 1 of the protein sequence is the beginning of the signal peptide of the CGTase and the deletion brings about the increase in the .gamma.-CGTase activity of the protein.

CGTases, qui, lorsqu'elles convertissent des substrats amidonés ou semblables à l'amidon en cyclodextrines, produisent de la gamma-CD dans une proportion plus élevée et continuent de présenter au moins 60 % de l'activité CGTasique spécifique totale de la CGTase de départ utilisée pour préparer l'enzyme en question. Les séquences d'acides aminés diffèrent de celles des CGTases connues par la délétion d'au moins un acide aminé dans la région allant de la position 155 jusqu'à la position 195 inclusivement, la position 1 de la séquence de la protéine étant le début du peptide signal de la CGTase, et la délétion entraîne une augmentation de l'activité gamma-CGTasique de la protéine.

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