Cyclomaltodextrin glucanotransferase variants

Details Cyclomaltodextrin glucanotransferase variants Cyclomaltodextrin glucanotransferase variants

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A21D 8/04 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2217876

The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically, the invention relates to a maethod of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.

L'invention porte sur les variants de la cyclomaltodextrine glucanotrasnférase (CGTase), et en particulier sur un procédé de modification de la liaison de substrat et/ou de la sélectivité produit d'un enzyme précurseur de CGTase. Elle porte également sur les variants de CGTase obtenus à partir d'un enzyme précurseur de CGTase par substitution, insertion et/ou suppression d'un ou de plusieurs résidus d'acides aminés se trouvant en position proche du substrat. Elle se rapporte en outre à des produits de recombinaison d'ADN codant les variants de CGTase, à des vecteurs d'expression, à des cellules hôtes et à des procédés permettant de fabriquer les variants de CGTase de l'invention.

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