Cyp450-specific dna probes and primers, and biological...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C07H 21/04 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2450389

The present application relates to a set of new cDNA probes which enables the specific and simultaneous detection of the main fourteen CYP450 isoforms, and to new primers specific to the probes. It also provides transfection vectors, genetically engineered cells, and with amplification products derived therefrom. The present application notably encompasses any solid surface such as DNA filters or DNA chips which comprises a cDNA probe or an amplification product of the invention. The products provided thereby enables to evaluate the toxicity or pathogenicity of a product, and to predict drug in vivo interactions or efficiency.

L'invention concerne un ensemble de nouvelles sondes ADNc permettant la détection spécifique et simultanée des quatorze principales isoformes CYP450 et de nouvelles amorces spécifiques aux sondes. L'invention concerne également des vecteurs de transfection, des cellules conçues génétiquement et des produits d'amplification dérivés de ceux-ci. L'invention concerne en particulier toute surface solide telle qu'une puce ADN ou un filtre ADN comportant une sonde ADNc ou un produit d'amplification de la présente invention. Les produits ainsi obtenus permettent d'évaluer la toxicité ou la pathogénicité d'un produit et de prévoir les interactions des médicaments in vivo ou encore leur efficacité.

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