Cytochrome p450 monooxygenases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2282809

Cytochrome P450II dependent monooxygenases and DNA molecules encoding these monooxygenases are provided, which are able to catalyze the biosynthetic conversion of aldoximes to nitrils and the conversion of said nitrils to the corresponding cyanohydrins, which are the precursors of cyanogenic glycosides. Moreover, the invention provides methods for obtaining DNA molecules according to the invention and methods for obtaining transgenic plants resistant to insects, acarids, or nematodes or plants with improved nutritive value.

L'invention concerne des monooxygénases dépendantes du cytochrome P450II et des molécules d'ADN codant ces monooxygénases qui sont capables de catalyser la transformation biosynthéthique d'aldoximes en nitriles et la transformation desdits nitriles en cyanhydrines correspondantes qui sont les précurseurs des glucosides cyanogénétiques. En outre, l'invention concerne des procédés d'obtention de molécules d'ADN selon l'invention et des procédés d'obtention de plantes transgéniques qui résistent aux insectes, aux acariens ou aux nématodes ou de plantes à valeur nutritionnelle améliorée.

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