Cytokine expressed by dia/lif-deficient embryonic stem cells...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/52 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12N 15/01 (2006.01) C12P 21/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2246712

A novel cytokine having the capacity to inhibit differentiation of embryonic stem (ES) cells is provided. The cytokine is designated ESRF and is characterised by the capacity to inhibit differentiation of ES cells (i) in the absence of DIA/LIF and (ii) in the absence of cytokines which act through gp 130 and (iii) in the absence of interaction with gp 130 and (iv) in the absence of interaction with LIF receptor. The invention further provides methods of deriving and propagating ES cells, ES cells per se and cell lines useful in deriving and assaying novel factors.

L'invention concerne une nouvelle cytokine ayant la capacité d'inhiber la différenciation des cellules souches (ES). La cytokine est appelée ESRF et se caractérise par sa capacité à inhiber la différenciation des cellules ES (i) en l'absence de DIA (activité inhibant la différenciation)/LIF(facteur inhibiteur de la leucémie myéloïde), (ii) en l'absence de cytokines qui agissent par l'intermédiaire de la gp130, (iii) en l'absence de l'interaction avec la gp130, et (iv) en l'absence de l'interaction avec le récepteur de LIF. L'invention concerne également des procédés de dérivation et de propagation des cellules ES, des cellules ES elles-mêmes et des lignées cellulaires utilisées dans la dérivation et le dosage de nouveaux facteurs.

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