Cytokinin oxidase sequences and methods of use

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/06 (2006.01) C12N 15/82 (2006.01) C07K 14/415 (2006.01)

Patent

CA 2563344

Methods and compositions for modulating plant development are provided. Polynucleotide sequences and amino acid sequences encoding cytokinin oxidase polypeptides are provided. The sequences can be used in a variety of methods including modulating root development, modulating floral development, modulating leaf and/or shoot development, modulating seed size and/or weight, modulating tolerance under abiotic stress, and modulating resistance to pathogens. Polynucleotides comprising CKX promoters are also provided. The promoters can be used to regulate expression of a sequence of interest. Transformed plants, plant cells, tissues, and seed are also provided.

L'invention concerne des procédés et des compositions destinés à moduler les développements d'une plante. Les séquences polynucléotides et des séquences d'acide aminé codant des polypeptides de cytokinine oxydase sont produits. Les séquences peuvent être utilisées dans divers procédés consistant à moduler le développement de racines, à moduler le développement floral, à moduler le développement des feuilles et/ou des pousses, à moduler la dimension et/ou le poids des semences, à moduler la tolérance à des stress abiotiques, à moduler la résistance à des pathogènes. L'invention concerne également des polynucléotides contenant des promoteurs CKX. Ces promoteurs peuvent être utilisés afin de réguler l'expression d'une séquence d'intérêt. L'invention concerne également les plantes transformées, les cellules végétales, les tissus et les semences.

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