C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/31 (2006.01) C07K 14/205 (2006.01) C07K 16/12 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)
Patent
CA 2548381
With respect to unknown CDT genes of C. coli and C. fetus, cloning and sequence determination have been successfully accomplished. Further, C. jejuni and C. fetus CDT comparison has been carried out, and primers specific to and common to both have been developed. It has been probed that the developed primers are applicable to the multiplex PCR method capable of simultaneously conducting easy and speedy determination of the presence of CDT of Campylobacter genus bacteria and identification of bacterial strain thereof and can further be utilized in the typing according to the PCR-RFLP method.
L'invention concerne la détermination d'une séquence et le clonage de gènes CDT, jusque là inconnu, de C. coli et de C. fetus. En outre, la comparaison de CDT C. jejuni et de C. fetus est effectuée, et des amorces spécifiques à un des ces gènes ou communes aux deux gènes sont développées. Les amorces développées peuvent servir dans des applications de la méthode PCR multiplex permettant d'effectuer simultanément une détermination facile et rapide de la présence de CDT de bactéries de gènes campylobacter et l'identification d'une souche bactérienne de ce gène, et peuvent également être utilisées dans le typage, selon la méthode PCR-RFLP.
Asakura Masahiro
Yamasaki Shinji
Fuso Pharmaceutical Industries Ltd.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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