Cytoplasmic inhibition of gene expression and expression of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C07K 14/08 (2006.01)

Patent

CA 2434847

The present invention relates to recombinant viral vectors encoding a transcriptional unit, that encodes a fusion protein, or a foreign protein or a gene of interest to be silenced, which can be expressed in a host. The present invention also relates to the use of these recombinant viral vectors to express a fusion protein, a foreign protein, to silence a gene of interest in a host. The present invention also relates to the use of these recombinant viral vectors to screen a cDNA or genomic library in order to correlate a nucleotide sequence with a phenotypic or biochemical change.

L'invention concerne des vecteurs viraux recombinés codant pour une unité de transcription qui code pour une protéine hybride, une protéine étrangère ou un gène spécifique devant être inactivé, pouvant être exprimés dans un hôte. L'invention concerne également l'utilisation de ces vecteurs viraux recombinés pour l'expression d'une protéine hybride, d'une protéine étrangère, ou pour inactiver un gène spécifique dans un hôte. L'invention concerne également l'utilisation de ces vecteurs viraux recombinés pour le criblage d'un ADNc ou d'une banque génomique afin d'établir une corrélation entre une séquence nucléotidique et une modification phénotypique ou biochimique.

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