Cytoplasmic male sterility system producing canola hybrids

A - Human Necessities – 01 – H

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A01H 1/02 (2006.01) A01H 1/04 (2006.01) A01H 1/06 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2198125

Our invention comprises a gene restorer line of Brassica napus which contains a Raphanus sativus restorer gene but is essentially free of Raphanus sativus genes which produce high glucosinolate. In particular, we provide a gene restorer line, and progeny derived therefrom, seed of which is low in glucosinolates. The Brassica napus restorer lines are free of glucosinolate- producing genes having a characteristic RFLP signature. The method of producing such lines which comprises crossing Brassica napus restorer lines and hybrids with desired Brassica napus germplasm and selecting progeny having a characteristic RFLP signature is also encompassed by the present invention.

L'invention concerne une lignée de Brassica napus qui contient un gène restaurateur de Raphanus sativus, mais qui est sensiblement exempte de gènes de Raphanus sativus produisant de grandes quantités de glucosinolate. Plus particulièrement, l'invention concerne une lignée avec ce gène restaurateur, ses graines et sa descendance, produisant peu de glucosinolates. Les lignées de Brassica napus contenant le gène restaurateur sont exemptes de gènes produisant du glucosinolate et elles ont la signature caractéristique RFLP. Le procédé selon l'invention pour produire ces lignées consiste à croiser des lignées de Brassica napus contenant un gène restaurateur et des hybrides avec le germoplasme choisi de Brassica napus, puis à sélectionner la descendance ayant la signature caractéristique RFLP.

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