Cytoplasmic transfer to de-differentiate recipient cells

Details Cytoplasmic transfer to de-differentiate recipient cells Cytoplasmic transfer to de-differentiate recipient cells

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C12N 5/071 (2010.01) C12N 5/073 (2010.01) C12N 5/075 (2010.01) C12N 15/873 (2010.01) C12N 5/12 (2006.01) C12N 5/16 (2006.01) C12N 5/22 (2006.01) C12N 5/26 (2006.01) C12N 5/28 (2006.01) C12N 15/02 (2006.01) C12N 15/07 (2006.01) C12N 15/08 (2006.01) C12N 15/09 (2006.01) C12N 15/87 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2377515

Methods for de-differentiating or altering the life-span of desired "recipient" cells, e.g., human somatic cells, by the introduction of cytoplasm from a more primitive, less differentiated cell type, e.g., oocyte or blastomere are provided. These methods can be used to produce embryonic stem cells and to increase the efficiency of gene therapy by allowing for desired cells to be subjected to multiple genetic modifications without becoming senescent. Such cytoplasm may be fractionated and/or subjected to subtractive hybridization and the active materials (sufficient for de-differentiation) identified and produced by recombinant methods.

L'invention concerne des techniques permettant de réduire la différentiation de cellules receveuses désirées, ou d'altérer leur durée de vie, par exemple des cellules somatiques humaines, par l'introduction d'un cytoplasme d'un type de cellule moins différenciée, plus primitive, par exemple un oocyte ou un blastomère. On peut utiliser ces techniques pour produire des cellules souches embryonnaires, et pour augmenter l'efficacité d'une thérapie génique par soumission des cellules désirées à plusieurs modifications génétiques sans que celles-ci deviennent sénescentes. Ce cytoplasme peut être fractionné, et/ou soumis à une hybridation soustractive, et les matériaux actifs (suffisants pour réduire la différentiation) sont identifiés et produits au moyen de techniques de recombinaison.

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