G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 1/28 (2006.01) G01N 33/483 (2006.01)
Patent
CA 2106343
Abstract A process for preparing a monolayer of cytological material on a cationically-charged substrate includes the steps of separating the cytological material by centrifugation over a density gradient, producing a packed pellet of the cytological material, mixing the pellet of the cytological material, withdrawing an aliquot of a predetermined volume of the material from the mixed pellet of the cytological material, depositing the aliquot and a predetermined volume of water into a sedimentation vessel, which is removably secured to the substrate, allowing the cytological material to settle onto the substrate under the force of gravity, and after settlement of the cytological material, removing the water from the sedimentation vessel.
Procédé de préparation d'une couche simple de matière cytologique sur un substrat positivement chargé comportant les étapes suivantes : séparer la matière cytologique par centrifugation au gradient de densité, produire un culot compact de matière cytologique, mélanger le culot obtenu, retirer du culot mélangé une partie aliquote d'un volume prédéterminé de matière cytologique, déposer la partie aliquote et un volume prédéterminé d'eau dans un récipient de sédimentation fixé de façon non permanente au substrat, permettant ainsi à lamatière cytologique de se déposer sur le substrat sous l'effet de la pesanteur et, une fois la matière cytologique déposée, enlever l'eau du récipient de sédimentation.
Carrico Charles Leo Jr.
Fox William Alan
Geyer James Winston
Knesel Ernest Arthur Jr.
Autocyte Inc.
Gowling Lafleur Henderson Llp
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