Cytotoxic antibodies targeting antibodies inhibiting factor...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/13 (2006.01) C12N 5/078 (2010.01) A61K 39/395 (2006.01) A61P 7/04 (2006.01) C07K 16/42 (2006.01) C07K 16/46 (2006.01) C12N 5/10 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2638166

The invention relates to an anti-idiotypical antibody targeting an antibody inhibiting the human factor VIII, said inhibiting antibody targeting the C2 region of the human factor VIII, the variable region of each of the light chains thereof being encoded by a sequence of nucleic acids of which at least 70 % is identical to the murine sequence of nucleic acids SEQ ID NO: 1, and the variable region of each of the heavy chains thereof being encoded by a sequence of nucleic acids of which at least 70 % is identical to the murine sequence of nucleic acids SEQ ID NO: 2, the constant regions of the light chains and the heavy chains being constant regions from a non-murine species. The invention also relates to the use of said antibody for activating the Fc?RIII receptors of cytotoxic immune cells, and to the production of a medicament especially for the treatment of haemophilia A.

La présente invention se rapporte à un anticorps anti-idiotypique dirigé contre un anticorps inhibiteur du facteur VIII humain, ledit anticorps inhibiteur étant dirigé contre la région C2 du facteur VIII humain, dont la région variable de chacune des chaînes légères est codée par une séquence d'acides nucléiques possédant au moins 70% d'identité avec la séquence d'acides nucléiques murine SEQ ID NO : 1, la région variable de chacune de ses chaînes lourdes est codée par une séquence d'acides nucléiques possédant au moins 70% d' identité avec la séquence d'acides nucléiques murine SEQ ID NO : 2, et dont les régions constantes des chaînes légères et des chaînes lourdes sont des régions constantes provenant d'une espèce non-murine, ainsi qu'à l'utilisation de cet anticorps pour activer les récepteurs Fc?RIII de cellules immunitaires cytotoxiques, et pour la fabrication d'un médicament, notamment destiné au traitement de l'hémophilie A.

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