Cytotoxic t lymphocytes activated by dendritic cell hybrids

C - Chemistry – Metallurgy – 12 – N

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C12N 5/16 (2006.01) A61K 39/00 (2006.01) C12N 5/00 (2006.01) C12N 5/12 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2399432

Methods of generating CTL using fused cells formed by fusions between dendritic cells and non-dendritic cells (e.g., cancer cells), CTL generated by these methods, methods of treatment involving administering the CTL to mammalian subjects, methods of identifying peptides recognized by the CTL, and methods of inducing immune response in mammalian subjects involving administration of the identified peptides, or polypeptide antigens of which the peptides are fragments, to the mammals.

La présente invention concerne des procédés permettant de produire des lymphocytes T cytotoxiques, par utilisation de cellules fusionnées, qui sont formées par fusion entre des cellules dendritiques et des cellules non dendritiques (telles que des cellules cancéreuses). La présente invention concerne également des lymphocytes T cytotoxiques produits selon ces procédés, des procédés de traitement impliquant l'administration desdits lymphocytes T cytotoxiques à des sujets mammifères, des procédés d'identification de peptides reconnus par lesdits lymphocytes T cytotoxiques, ainsi que des procédés d'induction de réponse immunitaire chez des sujets mammifères, impliquant l'administration desdits peptides identifiés ou d'antigènes de polypeptides, dont lesdits peptides sont des fragments, auxdits mammifères.

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