Cytotoxin-based biological containment

Details Cytotoxin-based biological containment Cytotoxin-based biological containment

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2327387

Method of conditionally controlling the survivability of a recombinant cell population and of containing such cells to an environment or containing replicons to a host cell is based on the use of proteic killer systems including the E. coli relBE locus and similar systems found in Gram-negative and Gram-positive bacteria and Archae. Such system are generally based on a cytotoxin polypeptide and an antitoxin or antidote polypeptide that in contrast to the cytotoxin is degradable by proteases. The recombinant cells are useful as vaccines, pollulant degrading organisms or as biogical pest control organisms e.g. expressing B. thuringiensis crystalline proteins.

On décrit un procédé pour réaliser un contrôle conditionnelle de la pérennité d'une population de cellules recombinantes et pour confiner lesdites cellules dans un environnement ou confiner des réplicons dans une cellule hôte. Le procédé est fondé sur l'utilisation de systèmes protéiques lytiques comprenant le locus E. coli relBE et d'autres systèmes présents dans des bactéries gram positif ou gram négatif ou dans Archae. De tels systèmes sont généralement basés sur un polypeptide cytotoxine ou un polypeptide antitoxine ou antidote qui, à la différence de la cytotoxine, peut se décomposer sous l'action de protéases. Les cellules recombinantes peuvent s'utiliser comme vaccins, comme organismes de dégradation de polluants, ou comme organismes biologiques antiparasitaires (p. ex. exprimant les protéines cristallines B. thuringiensis).

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