D-amino acid oxidase and method for isolation thereof

Details D-amino acid oxidase and method for isolation thereof D-amino acid oxidase and method for isolation thereof

C - Chemistry, Metallurgy

12

N

195/123, 195/39,

C12N 9/06 (2006.01) C12N 11/10 (2006.01)

Patent

CA 1283614

ABSTRACT A Trigonopsis variabilis D-amino acid oxidase in substantially pure form and active against cephalo- sporin C is disclosed. This D-amino acid oxidase is isolated from Trigonopsis variabilis by a method which is performed in three steps, namely: (a) acidifying and heating a crude cell extract of Triqonopsis variabilis to obtain a precipitate and supernatant fraction; (b) treating said supernatant fraction obtained in step (a) with sufficient ammonium sulfate to obtain a second precipitate, said second precipitate con- taining the D-amino acid oxidase of claim 1; and (c) resuspending the precipitate obtained in step (b) and collecting the D-amino acid oxidase by isoelectric precipitation.

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