Data analysis and display system for ligation-based dna...

Details Data analysis and display system for ligation-based dna... Data analysis and display system for ligation-based dna...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) G06F 17/00 (2006.01)

Patent

CA 2388738

A data analysis system for ligation-based sequencing is provided. The system includes a base calling algorithm that may be implemented with a program of instructions (e.g., software) for determining a signature of a nucleotide sequence. A graphical user interface (GUI) enables a user to interact with and control various aspects of the base calling algorithm. The base calling algorithm and associated software may be used in connection with a technique that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 µm diameter microbeads.

L'invention concerne un système d'analyse de données servant à effectuer un séquencement à base d'une ligation. Ce système comprend un algorithme d'appel de base pouvant être mis en application avec un programme d'instructions, (par exemple, logiciel) servant à déterminer une signature de séquence de nucléotides. Une interface d'utilisateur graphique (GUI) permet à l'utilisateur d'entrer en interaction avec cet algorithme d'appel de base et d'en contrôler différents aspects. On peut utiliser cet algorithme et le logiciel associé en association avec une technique combinant un séquencement de signature exempt de base de gel avec un clonage in vitro de millions de gabarits sur des microbilles séparées de 5 µm de diamètre.

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