Data-dependent selection of dissociation type in a mass...

H - Electricity – 01 – J

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H01J 49/00 (2006.01)

Patent

CA 2657809

Methods and apparatus for data-dependent mass spectrometric MS/MS or MSn analysis are disclosed. The methods may include determination of the charge state of an ion species of interest, followed by automated selection of a dissociation type (e.g., CAD, ETD, or ETD followed by a non-dissociative charge reduction or collisional activation) based at least partially on the determined charge state. The ion species of interest is then dissociated in accordance with the selected dissociation type, and an MS/MS or MSn spectrum of the resultant product ions may be acquired.

L'invention concerne des procédés et un appareil pour une analyse MS/MS ou MSn spectrométrique de masse dépendante des données. Les procédés peuvent comprendre la détermination de l'état de charge d'une espèce d'ion intéressante, suivie par la sélection automatisée d'un type de dissociation (par exemple, CAD, ETD ou ETD suivi par une réduction de charge non dissociative ou une activation par collision) sur la base au moins partiellement de l'état de charge déterminé. L'espèce d'ion intéressante est ensuite dissociée en accord avec le type de dissociation sélectionné, et un spectre MS/MS ou MSn des ions produits résultants peut être acquis.

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