De novo cell-free synthesis of picornavirus

Details De novo cell-free synthesis of picornavirus De novo cell-free synthesis of picornavirus

C - Chemistry, Metallurgy

12

N

C12N 7/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2115991

2115991 9300442 PCTABS00019 A process for the de novo synthesis of a picornavirus using a cell-free medium has been developed. The cell-free medium is prepared from a lysate of mammalian cells from which the nuclei and mitochondria has been removed, endogenous mRNA deactivated and supplemented with nucleoside triphosphates, tRNA, amino acids and precursors necessary for generating proteins. The genomic RNA of the picornavirus or rhinovirus is added to the cell-free medium and after incubation at about 30 ·C to 40 ·C of from about 4 to 24 hours infectious, mature virus particules are formed.

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