Deacetoxycephalosporin c hydroxylase

Details Deacetoxycephalosporin c hydroxylase Deacetoxycephalosporin c hydroxylase

C - Chemistry, Metallurgy

12

N

195/78, 195/97,

C12N 9/18 (2006.01) C12N 9/00 (2006.01) C12P 35/06 (2006.01)

Patent

CA 2026041

Deacetoxycephalosporin C hydroxylase is obtained in purified form from crude cell-free extracts via chromatography over a weak anionic exchange resin, ammonium sulfate fractionation, gel filtration, hydroxylapatite chromatography, and FPLC. The enzyme is obtained in >90% purity by further gel filtration and a second FPLC. The 28-residue amino-terminal sequence of hydroxylase is provided as well as a 9-residue amino-terminal sequence of an internal sequence and a 3-residue carboxy-terminal sequence. Tn addition to the efficient conversion of DAOC to DAC, the hydroxylase provided herein converts 7.beta.-(.alpha.-amino- adipamido)-3-examethylenecepham-4-carboxylic acid to DAC.

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