Deactivation of the inner surfaces of capillaries

Details Deactivation of the inner surfaces of capillaries Deactivation of the inner surfaces of capillaries

G - Physics

01

N

G01N 27/447 (2006.01) B01D 57/02 (2006.01)

Patent

CA 2105821

The invention relates to a process for deactivating the inner surfaces of capillaries for capillary zone electrophoresis and capillary gel electrophoresis. The deactivation is effected by coating said inner surface with polar polymers which initially are water-soluble, whereupon the polymers are subsequently thermally fixed by a formation of water-insoluble polymers. The invention further relates to the capillaries thus produced and to the use thereof for the separation of oligonucleotides, peptides and proteins.

L'invention concerne un procédé pour désactiver les surfaces internes des capillaires pour l'électrophorèse de zone sur colonne capillaire et l'électrophorèse sur gel en capillaire. La désactivation est effectuée par le revêtement de ladite surface interne avec des polymères polaires qui initialement sont hydrosolubles, après quoi les polymères sont fixés par la chaleur par la formation de polymères insolubles dans l'eau. L'invention concerne également les capillaires ainsi produits et l'utilisation de ces derniers pour la séparation des oligonucléotides, peptides et protéines.

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