Debranching enzymes and dna sequences coding them, suitable...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) A01H 5/00 (2006.01) A23L 1/0522 (2006.01) C12N 9/44 (2006.01) C12N 15/82 (2006.01) C12P 19/16 (2006.01)

Patent

CA 2169174

DNA sequences are described which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants code new proteins with the enzymatic activity of debranching enzymes which in transgenic plants reduce the degree of branching of amylopectin starch and DNA sequences which on the codogenic strand code plant debranching enzymes, whose transcripts formed in transgenic plants prevent the synthesis of proteins with the enzymatic activity of debranching enzymes, which in the transgenic plants increases the degree of branching of amylopectin starch, as well as plasmids on which these DNA sequences are localized, which can be introduced into plant cells and plants. Also described is a process for the production of plants changed by genetic engineering whose amylopectin starch is modified, and the modified starch obtainable from these plants.

L'invention concerne des séquences d'ADN codant, sur le brin codogénique, des enzymes de déramification végétale dont les transcrits formés dans les plantes transgéniques codent de nouvelles protéines présentant l'activité enzymatique des enzymes de déramification pouvant réduire le degré de ramification de l'amidon amylopectinique dans des plantes transgéniques; des séquences d'ADN codant, sur le brin codogénique, des enzymes de déramification végétale dont les transcrits formés dans des plantes transgéniques empêchent la synthèse des protéines présentant l'activité enzymatique des enzymes de déramification pouvant augmenter le degré de ramification de l'amidon amylopectinique dans les plantes transgéniques; et des plasmides pouvant être introduits dans les cellules végétales et les plantes, lesdites séquences d'ADN étant situées sur ces plasmides. On a également prévu un procédé de production de plantes manipulées génétiquement dont l'amidon amylopectinique est modifié, et l'amidon modifié obtenu à partir de ces plantes.

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