Defective recombinant retrovirus and its use for insertion...

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.2, 195/1.2

C12N 15/00 (2006.01) C12N 15/867 (2006.01)

Patent

CA 1341258

L'invention concerne un retrovirus recombinant défectif contenant une sequence codante pour une protéine déterminée dans le génome des cellules. L'ADN du rétrovi- rus recombinant contient la séquence codante pour la pro- téine intercalée entre un site accepteur d'épissage et un site donneur d'épissage, l'ensemble étant place sous le contrôle d'un promoteur approprié, et ceci dans des condi- tions telles que l'infection desdites cellules par ce ré- trovirus recombinant s'accompagne de l'incorporation de la séquence codante pour la protéine dans le génome des cel- lules infectées.

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