Defined cell culturing surfaces and methods of use

Details Defined cell culturing surfaces and methods of use Defined cell culturing surfaces and methods of use

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C12N 5/07 (2010.01) C12M 3/00 (2006.01)

Patent

CA 2674111

In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.

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