Defined culture medium for cho cells

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/02 (2006.01) C07K 16/28 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2053586

A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cell lines, which is essentially free from protein, lipid and carbohydrate isolated from an animal source, comprising water, an osmolality regulator, a buffer, an energy source, amino acids including L-glutamine, an inorganic or recombinant iron source, and a synthetic or recombinant growth factor, and optionally non-ferrous metal ions vitamins and cofactors; also cells adapted to grow in such a culture medium.

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