C - Chemistry – Metallurgy – 07 – H
Patent
C - Chemistry, Metallurgy
07
H
260/229, 195/1.1
C07H 19/16 (2006.01) C07H 19/06 (2006.01) C07H 21/00 (2006.01)
Patent
CA 1231650
Defined Sequence Single Strand Oligonucleotides Incorporating Reporter Groups, Process for the Chemical Synthesis Thereof, and Nucleosides Useful in Such Synthesis Abstract Defined sequence single strand oligonucleotides which have a length of fewer than 200 units, incor- porate modified nucleotide units which are pyrimidine or purine-based, and which may have readily detectable reporter groups attached to substituents on the modified nucleotide units thereof, are disclosed. Each reporter group is attached to the base of the respective nucleo- tide unit at a sterically tolerant site thereon, exemplified by the C-5 position of pyrimidine-based nucleotides and the C-8 position of purine-based nucleotides. Such oligonucleotides are useful in identification, localization, isolation and/or detection of complementary nucleic acid sequences of interest in cellular or cell-free systems. Also disclosed is a process for the chemical synthesis of single strand oligonucleotides having a predeter- mined number of modified pyrimidine and/or purine- based nucleotide units in predetermined sequence, to the bases of which readily detectable reporter groups are attached at sterically tolerant sites through substituents thereon, either prior to or after incorporation of such units into the oligo- nucleotide chain. Novel nucleosides useful in the chemical synthesis of labeled, defined sequence single strand oligonucleotides are also disclosed.
460489
Molecular Biosystems Inc.
Osler Hoskin & Harcourt Llp
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