Deglycosylated enzymes for conjugates

Details Deglycosylated enzymes for conjugates Deglycosylated enzymes for conjugates

C - Chemistry, Metallurgy

12

N

C12N 9/96 (2006.01) C12N 9/16 (2006.01) G01N 33/535 (2006.01)

Patent

CA 2515686

If an enzyme derived from a eukaryotic organism is deglycosylated prior to forming a conjugate, i.e. prior to attaching the enzyme to a molecule capable of binding to a target molecule, the sensitivity of an assay for detecting the presence or determining the quantity of a target molecule, in which the conjugate is used as a labelled component, can be increased. The present invention provides conjugates, methods for providing the same, use of such conjugates as well as kits containing the conjugates.

Si une enzyme dérivée d'un organisme eucaryotique est déglycosylée avant de former un conjugué, c.-à-d. avant la fixation de l'enzyme à une molécule capable de se lier à une molécule cible, la sensibilité d'un dosage, destiné à détecter la présence ou déterminer la quantité d'une molécule cible dans laquelle le conjugué est utilisé comme composant marqué, peut être accrue. La présente invention porte également sur des conjugués, sur leur procédé de production, sur leur utilisation ainsi que sur les kits les contenant.

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