Degradation and detection of prion infectivity

Details Degradation and detection of prion infectivity Degradation and detection of prion infectivity

C - Chemistry, Metallurgy

12

N

C12N 9/52 (2006.01) A61K 38/48 (2006.01) A61L 2/16 (2006.01) A61L 2/18 (2006.01) A61L 2/22 (2006.01) A61L 9/02 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01) C11D 3/386 (2006.01) C11D 3/48 (2006.01) C12N 9/54 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2434129

A transmissible spongiform encephalopathy (TSE) agent is inactivated by exposing the TSE agent to a thermostable proteolytic enzyme at elevated temperature and at acid or alkaline pH. Following this step, or separately, presence of TSE infectivity is detected by detection of dimers of prion protein.

L'invention concerne un agent de l'encéphalopathie spongiforme transmissible (TSE) inactivé par exposition de l'agent TSE à une enzyme protéolytique thermostable à température élevée et à pH acide ou alcalin. Suite à cette étape, ou séparément, la présence de l'infectivité TSE est détectée par détection de dimères de la protéine prion.

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