Degradation of nucleic acid by peroxides to reduce viscosity

Details Degradation of nucleic acid by peroxides to reduce viscosity Degradation of nucleic acid by peroxides to reduce viscosity

C - Chemistry, Metallurgy

12

N

C12N 1/08 (2006.01) C12P 7/62 (2006.01)

Patent

CA 2146455

Peroxide, particularly supplied as hydrogen peroxide, is an effective nucleic acid degrading agent and can be used in the recovery of intracellularly produced materials from cell (particularly bacterial) lysates. The degradation or removal of nucleic acids from cell lysates is important because they form solutions of high viscosity which interfere with subsequent processing. Peroxide degradation is particularly useful in the recovery of polyhydroxyalkanoate polymers such as polyhydroxybutyrate/valerate from lysates of bacterial cells in which they are produced.

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