C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 13/00 (2006.01) C08G 69/48 (2006.01) C08G 73/02 (2006.01) C12N 1/20 (2006.01) C12P 5/00 (2006.01) C12P 7/04 (2006.01) D21H 17/55 (2006.01) D21H 21/20 (2006.01)
Patent
CA 2066378
Enzymatic dehalogenation of undesirable nitrogen-free organohalogen compounds is carried out by adding a dehalogenase to an aqueous composition comprising a nitrogen-free organohalogen compound and a nitrogen-containing cationic polymer. The enzymatic dehalogenation of the nitrogen-free organohalogen compound is achieved without any substantial effect upon the nitrogen-free organohalogen compound. The dehalogenation method may be carried out via continuous, batch, or semi-continuous processes. The composition is especially useful as a paper wet strengthening formulation, and is useful in commercial papermaking processes. The resulting paper product comprises a residue of a microorganism. Preferably the enzyme is obtained from a biocatalyst, still more preferably from a microorganism. Batch-enrichment of a culture of a dehalogenase-containing microorganism provides a composition capable of substantially eliminating the presence of nitrogen-free organohalogen compounds, which are considered to be pollutants. Cell concentrations of greater than 5 x 10 7 cells/ml are provided.
Bull Alan
Hardman David J.
Stubbs Brian M.
Hercules Incorporated
Moffat & Co.
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