Dehydrated immediate reconstruction culture medium to...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/14 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2336956

The invention relates to a dehydrated culture medium for detecting <i>Streptococcus agalactiae</i> by their pigment, wherein only water is added and the medium is then shaken prior to utilization. Once rehydrated, a semisolid gel is produced which enables growth of <i>S. agalactiae</i> inside the medium as pigmented colonies thereby facilitating easy detection. The new instant reconstruction medium consists of a dehydrated culture medium and a gel-forming agent. When the user adds ambient temperature water and shakes the medium, a gel is immediately formed and the medium is then ready for utilization. <i>Streptococcus agalactiae</i> grows in the medium as pigmented colonies thereby enabling easy detection thereof. Once rehydrated, the medium has sufficient consistency for the bacterial growth not to form sediments and solidifies rapidly enough so that air bubbles are trapped when the medium is shaken. Said air bubbles remain inside the medium during the incubation period and create interphases activating the production of S. agalactiae pigment producing heavily pigmented colonies.

L'invention concerne un milieu de culture déshydraté permettant de détecter des <i>Streptococcus agalactiae</i> grâce à leur pigment et ce en ajoutant de l'eau et en la mélangeant au milieu. Lors de sa réhydration, le milieu de culture produit un gel semisolide permettant la croissance de <i>S.agalactiae</i> sous forme de colonies pigmentées facilitant leur détection. Le nouveau milieu à reconstruction instantanée est constitué d'un milieu de culture déshydraté et d'un agent gélifiant. Lorsque l'utilisateur ajoute de l'eau à température ambiante et qu'il agite le mélange, un gel se forme immédiatement et le milieu est prêt à être utilisé. Les <i>Streptococcus agalactiae</i> prolifèrent dans le milieu sous forme de colonies pigmentées facilitant leur détection. La réhydration confère à ce milieu une consistance suffisante pour que la croissance bactérienne ne se dépose comme sédiment et elle se solidifie suffisamment vite pour que des bulles d'air soient piégées après agitation. Ces bulles restent dans le milieu pendant l'incubation et créent des interphases activant la production de pigment des <i>S. agalactiae</i> générant ainsi des colonies fortement pigmentées.

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