Delayed solid phase immunologic assay

Details Delayed solid phase immunologic assay Delayed solid phase immunologic assay

G - Physics

01

N

167/139, 167/44

G01N 33/577 (2006.01) G01N 33/538 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1286605

ABSTRACT OF THE DISCLOSURE A method for the determination of a substance present in a sample, which comprises: (a) contacting a sample containing a substance with: (i) a first immunological binding partner to the substance, wherein the first immuno- logical binding partner is bound to biotin or a biotin-binding protein, (ii) a second immunological binding partner to the substance, wherein the second immu- nological binding partner is detectably labeled, and (iii) a biotin binding protein or biotin bound to a carrier; (b) incubating the components of step (a) for a period of time and under conditions suffi- cient to form an immune complex between the substance, the first immunological binding partner, the second immunological binding partner, and the carrier; (c) separating the carrier from the sample; and (d) determining the detectably labeled second immunological binding partner in either the sample or the carrier; wherein the reaction between the first immunologi- cal binding partner and the carrier occurs after, or substantially contemporaneously with, formation of an immune complex between the first immunological binding partner and the substance.

508188

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