Deletion mutants as vaccines for cholera

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/20 (2006.01) A61K 39/106 (2006.01) C07K 14/28 (2006.01) C12N 1/21 (2006.01) C12N 1/36 (2006.01)

Patent

CA 2139655

The invention features of nontoxigenic, genetically stable mutant strains of V cholerae and a method of making which are useful as live, oral vaccines for inducing immunological protection against cholera. The mutant strains are genetically engineered mutants which lack DNA encoding a functional ctxA subunit which is responsible for many of the symptoms of cholera. The strains also lack any functional attRS1 sequences which are required for recombination and amplification of the CTX genetic element. These strains are safe because they can not recombine with the wild type attRS1-containing vehicles which include the ctxA-encoding DNA.

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