Delignifying preparation exhibiting...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/24 (2006.01) C07H 21/00 (2006.01) C12N 1/20 (2006.01) C12N 1/22 (2006.01) C12Q 1/68 (2006.01) C12S 3/08 (2006.01) D21C 9/10 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2132335

2132335 9320192 PCTABScor01 A preparation exhibiting enzymatic activity comprising a substantial portion of .alpha.-L-arabinofuranosidase activity having the capability of delignifying wood pulp at a pH of 8 - 9 and a temperature of 65 ·C, is disclosed. Said preparation is obtainable by aerobic fermentation of a Bacillus stearothermophilus strain selected from the deposited strain NCIMB 40494 and mutants and variants thereof. The deposited strain and mutants and variants thereof are comprised by the invention. A method of producing said preparation, a process comprising treatment of wood pulp with said preparation, and wood pulp that has been treated with said preparation, and also said preparation in association with a preparation exhibiting enzymatic activity produced by another microbial strain and having the capability of delignifying wood pulp at a temperature of at least 65 ·C and a pH of at least 9, are disclosed. Use of an enzyme expressed by the deposited strain and .alpha.-L-arabinofuranosidase which comprises one or two specified partial sequences and homologues thereof, are also included.

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