Delivery of dsrna to arthropods

Details Delivery of dsrna to arthropods Delivery of dsrna to arthropods

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01K 67/033 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2455490

The invention is to methods of gene silencing in arthropods using dsRNA. The method is includecontacting the arthropod with, and/or directly feeding the arthropod, the dsRNA to the arthropods to deliver the dsRNA to arthropod tissues. It is envisaged that the methods of the invention will have use in determining the biological function of genes in arthropods. Methods of pest control of arthropods, and of protecting arthropods against parasites and predators are provided. Transgenic arthropods expressing dsRNA molecules are also provided by the present invention.

La présente invention a trait à des procédés de silençage génique chez les arthropodes au moyen d'ARN bicaténaires. Le procédé comporte la mise en contact de l'arthropode avec les ARN bicaténaires et/ou l'alimentation direct aux arthropodes des ARN bicaténaires pour l'administration des ARN bicaténaires dans les tissus des arthropodes. Les procédés de l'invention peuvent être utilisés dans la détermination de la fonction biologique de gènes chez les arthropodes. L'invention concerne également des procédés de lutte antiparasitaire chez les arthropodes, et de protection des arthropodes contre les parasites et les prédateurs. L'invention concerne en outre des arthropodes transgéniques exprimant des molécules d'ARN bicaténaires.

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