Delivery of nucleic acids into eukaryotic genomes using in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/90 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2522438

The present invention relates to genetic engineering and especially to the use of DNA transposition complex of bacteriophage Mu. In particular, the invention provides a gene transfer system for eukaryotic cells, wherein in vitro assembled Mu transposition complexes are introduced into a target cell and subsequently transposition into a cellular nucleic acid occurs. The invention further provides a kit for producing insertional mutations into the genomes of eukaryotic cells. The kit can be used, e.g., to generate insertional mutant libraries.

La présente invention se rapporte au génie génétique et particulièrement à l'utilisation d'un complexe de transposition d'ADN du bactériophage Mu. En particulier, l'invention se rapporte à un système de transfert de gènes pour cellules eucaryotes, dans lequel des complexes assemblés <i>in vitro</i> de transposition de Mu sont introduits dans une cellule cible et dans lequel se produit ultérieurement une transposition en un acide nucléique cellulaire. L'invention se rapporte également à une trousse de production de mutations insertionnelles dans les génomes de cellules eucaryotes. Cette trousse peut être utilisée, par exemple, pour générer des bibliothèques de mutants insertionnels.

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